ROCKVILLE, MD, June 29—EntreMed, Inc. (Nasdaq: ENMD), a clinical-stage pharmaceutical company developing therapeutics primarily for the treatment of cancer and inflammatory diseases, today announced that Duke University Medical Center has received a grant from the Susan G. Komen Breast Cancer Foundation to study the Company’s lead compound, 2ME2 (2-Methoxyestradiol or Panzem®), in metastatic breast cancer. Basic, translational and clinical studies will be conducted using Panzem® NCD in combination with a variety of chemotherapeutic agents to determine the effect of 2ME2 on chemotherapy resistant tumors. EntreMed will supply Panzem® NCD for these studies.

Kimberly L. Blackwell, M.D., Director, Clinical Trials in Breast Cancer at the Duke University Medical Center commented, “Our prior studies demonstrate that the inhibition of HIF-1αlpha causes near-complete destruction of tumor vessels and significantly improved radio-sensitivity in radiation resistant breast tumors. We believe that the inhibition of HIF-1αlpha may have the same or similar impact on chemotherapy-resistant tumors. Through studies performed under this grant, we hope to determine which chemotherapy agents and HIF- 1alpha inhibiting drugs work best together.”

Breast cancer remains a significant health problem, killing over 40,000 American women annually, despite the recent introduction of new therapies. Ten-year survival rates for women receiving standard systemic therapy is 50- 80% due to insufficient response to hormonal and chemotherapy. A primary resistance mechanism is thought to be the upregulation of HIF-1αlpha. 2ME2 is a potent inhibitor of HIF-1αlpha, and its down-regulation is closely associated with 2ME2’s tubulin inhibitor properties. HIF-1αlpha is over- expressed in more than 70% of human tumors and HIF-1αlpha over-expression correlates with tumor aggressiveness, metastases and poor prognosis.

Carolyn F. Sidor, M.D., M.B.A, EntreMed Vice President and Chief Medical Officer, commented on the grant, “Tumor chemoresistance continues to be a problem in metastatic breast cancer. The Duke University studies are designed to determine if blocking HIF-1αlpha will overcome cancer cell resistance to standard chemotherapeutic agents. We are pleased to have the opportunity to contribute to the research effort of this leading breast cancer research team.”

Dr. Sidor further commented, “EntreMed believes that 2ME2 has the potential to be a unique cancer therapy and may be particularly well-suited to attack tumors that are dependent on the pro-angiogenic activity of HIF-1αlpha. Panzem® NCD’s safety profile and lack of MDR chemoresistance offer opportunities for combining it with conventional chemotherapies to increase their effectiveness while decreasing toxicity. The results of the Duke studies will provide valuable information regarding the potential for Panzem® NCD to complement chemotherapy.”

Panzem® NCD is a novel anticancer agent, which is part of a next generation of antimitotic cancer drugs that bind to tubulin and work through multiple cellular pathways. 2ME2 can attack tumors on multiple fronts—directly by disrupting microtubules, an intracellular matrix necessary for the rapid division of cancer cells (mitosis), by inducing programmed cell death (apoptosis), and by blocking blood vessels that feed tumors (angiogenesis inhibition). EntreMed is currently evaluating 2ME2 formulations (Panzem® Capsules and Panzem® NCD) in Phase I and II clinical oncology studies.

About EntreMed

EntreMed, Inc. (Nasdaq: ENMD) is a clinical-stage pharmaceutical company developing therapeutic candidates primarily for the treatment of cancer and inflammation. Panzem® (2-methoxyestradiol or 2ME2), the Company’s lead drug candidate, is currently in clinical trials for cancer, as well as in preclinical development for non-oncology indications. EntreMed’s goal is to develop and commercialize new compounds based on the Company’s expertise in angiogenesis, cell cycle regulation and inflammation—processes vital to the treatment of cancer and other diseases, such as rheumatoid arthritis. Additional information about EntreMed is available on the Company’s website at http://www.entremed.com and in various filings with the Securities and Exchange Commission.

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